Identification of Cyanobacteria and Its Potential Toxins in the Joanes I Reservoir, Bahia, Brazil - MNHN - Muséum national d'Histoire naturelle Access content directly
Journal Articles Toxins Year : 2023

Identification of Cyanobacteria and Its Potential Toxins in the Joanes I Reservoir, Bahia, Brazil

Abstract

The Joanes I Reservoir is responsible for 40% of the drinking water supply of the Metropolitan Region of Salvador, Bahia, Brazil. For water sources such as this, there is concern regarding the proliferation of potentially toxin-producing cyanobacteria, which can cause environmental and public health impacts. To evaluate the presence of cyanobacteria and their cyanotoxins in the water of this reservoir, the cyanobacteria were identified by microscopy; the presence of the genes of the cyanotoxin-producing cyanobacteria was detected by molecular methods (polymerase chain reaction (PCR)/sequencing); and the presence of toxins was determined by liquid chromatography with tandem mass spectrometry (LC-MS/MS). The water samples were collected at four sampling points in the Joanes I Reservoir in a monitoring campaign conducted during the occurrence of phytoplankton blooms, and the water quality parameters were also analysed. Ten cyanobacteria species/genera were identified at the monitoring sites, including five potentially cyanotoxin-producing species, such as Cylindrospermopsis raciborskii, Cylindrospermopsis cf. acuminato-crispa, Aphanocapsa sp., Phormidium sp., and Pseudanabaena sp. A positive result for the presence of the cylindrospermopsin toxin was confirmed at two sampling points by LC-MS/MS, which indicated that the populations are actively producing toxins. The analysis of the PCR products using the HEPF/HEPR primer pair for the detection of the microcystin biosynthesis gene mcyE was positive for the analysed samples. The results of this study point to the worrisome condition of this reservoir, from which water is collected for public supply, and indicate the importance of the joint use of different methods for the analysis of cyanobacteria and their toxins in reservoir monitoring.
Fichier principal
Vignette du fichier
Pinheiro Menescal et al. - 2023 - Identification of Cyanobacteria and Its Potential .pdf (1.26 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive

Dates and versions

mnhn-04102635 , version 1 (22-05-2023)

Identifiers

Cite

Maria Teresa Araujo Pinheiro Menescal, Edna dos Santos Almeida, Emerson Andrade Sales, Annick Méjean, Claude Yéprémian. Identification of Cyanobacteria and Its Potential Toxins in the Joanes I Reservoir, Bahia, Brazil. Toxins, 2023, 15 (1), pp.51. ⟨10.3390/toxins15010051⟩. ⟨mnhn-04102635⟩
18 View
108 Download

Altmetric

Share

Gmail Facebook X LinkedIn More