This study examines individual commitment to biodiversity during adulthood. We studied the interrelations between everyday experiences of nature, knowledge about biodiversity, connectedness with nature, and implementation of specific pro-biodiversity practices, through a survey covering 473 adults in Paris surroundings (France). More specifically, we showed that people involved in experiences of nature in which attentiveness to biodiversity is explicit (citizen science, nature watch association, environmental association) have more knowledge about biodiversity and conservation than both people involved in experiences of nature in which attention to biodiversity remains implicit (community garden, allotment, community-supported agriculture), and people without such kinds of experience of nature. However, we found that people experiencing nature as part of a daily routine, whatever the type of experience, were more connected to nature and more likely to implement active pro-biodiversity practices. With this interdisciplinary study that links conservation biology and conservation psychology, we help understand more precisely the levels of commitment of urban and suburban adults toward biodiversity conservation. Citation: Prévot A.C., Cheval H., Raymond R. et Cosquer A. 2018. Routine experiences of nature in cities can increase personal commitment toward biodiversity conservation. Biological conservation 226: 1-8.