Revisiting the dynamics of cell wall synthesis in ovococci using click metabolic labeling and dSTORM imaging - Groupe Pneumocoque / Pneumococcus Group (IBS-PG)
Communication Dans Un Congrès Année : 2019

Revisiting the dynamics of cell wall synthesis in ovococci using click metabolic labeling and dSTORM imaging

Cécile Morlot

Résumé

Ovococci form a morphological group that includes several human pathogens (enterococci and streptococci). Their shape results from cell wall synthesis and remodeling processes that allow cell division and elongation. These processes are performed by multi-protein complexes that assemble into ring-like structures scaffolded by the cytoskeletal protein FtsZ at midcell. While most growth and division proteins are known, we still barely understand how they assemble into functional machineries in vivo, and how they coordinate their activities to ensure proper cell division and morphogenesis. Recently, we set up a combination of bioorthogonal labeling (using copper-free click chemistry) and dSTORM (direct STochastic Optical Reconstruction Microscopy) techniques to study cell wall synthesis in the human pathogen Streptococcus pneumoniae. I will present our approach and discuss how the nanoscale localization data it generates allow revisiting the current model for cell wall synthesis in ovococci.
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Dates et versions

hal-04870628 , version 1 (07-01-2025)

Identifiants

  • HAL Id : hal-04870628 , version 1

Citer

Cécile Morlot. Revisiting the dynamics of cell wall synthesis in ovococci using click metabolic labeling and dSTORM imaging. BMSV 2019 - Bacterial Morphogenesis, Survival and Virulence : Regulation in 4D, Nov 2019, Le Cap, South Africa. ⟨hal-04870628⟩
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